Automated Dynamic Resource Provisioning and Monitoring in Virtualized Large-Scale Datacenter

Infrastructure as a Service (IaaS) is a pay-as-you go based cloud provision model which on demand outsources the physical servers, guest virtual machine (VM) instances, storage resources, and networking connections. This article reports the design and development of our proposed innovative symbiotic simulation based system to support the automated management of IaaS-based distributed virtualized data enter. To make the ideas work in practice, we have implemented an Open Stack based open source cloud computing platform. A smart benchmarking application "Cloud Rapid Experimentation and Analysis Tool (aka CBTool)" is utilized to mark the resource allocation potential of our test cloud system. The real-time benchmarking metrics of cloud are fed to a distributed multi-agent based intelligence middleware layer. To optimally control the dynamic operation of prototype data enter, we predefine some custom policies for VM provisioning and application performance profiling within a versatile cloud modeling and simulation toolkit "CloudSim". Both tools for our prototypes' implementation can scale up to thousands of VMs, therefore, our devised mechanism is highly scalable and flexibly be interpolated at large-scale level. Autonomic characteristics of agents aid in streamlining symbiosis among the simulation system and IaaS cloud in a closed feedback control loop. The practical worth and applicability of the multiagent-based technology lies in the fact that this technique is inherently scalable hence can efficiently be implemented within the complex cloud computing environment. To demonstrate the efficacy of our approach, we have deployed an intelligible lightweight representative scenario in the context of monitoring and provisioning virtual machines within the test-bed. Experimental results indicate notable improvement in the resource provision profile of virtualized data enter on incorporating our proposed strategy

Flight investigation of the effects of Apollo heat-shield singularities on ablator performance

Launch vehicle flight tests of effects of Apollo heat shield irregularities on ablative material performanc

Intercellular trafficking and protein delivery by a herpesvirus structural protein

AbstractWe show that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin. VP22 movement was observed both after delivery of DNA by transfection or microinjection and during virus infection. Moreover, we demonstrate that VP22 trafficking occurs via a nonclassical Golgi-independent mechanism. Sensitivity to cytochalasin D treatment suggests that VP22 utilizes a novel trafficking pathway that involves the actin cytoskeleton. In addition, we demonstrate intercellular transport of a VP22 fusion protein after endogenous synthesis or exogenous application, indicating that VP22 may have potential in the field of protein delivery

The micro-optical ring electrode: Development of a novel electrode for photoelectrochemistry

The Micro-Optical Ring Electrode (MORE), a novel photoelectrochemical device capable of delivering light via a fibre optic light guide directly to the region of electrochemical measurement. Based on a ring microelectrode and using the insulating material interior to the ring electrode as a light guide, the MORE can therefore be used to conduct microelectrochemical studies of systems with complex photochemistry. Most recently, the MORE has been used in a preliminary study of the photoelectrochemical behaviour of tris(2,2'-bipyridine)ruthenium(II) (Ru(bipy)(3)(2+)) in the presence of the oxidising quenching agent, Fe3+. Chronoamperograms have been recorded at the MORE using increasing concentrations of Fe3+. A direct correlation has been established between the recorded photocurrent and [Fe3+]. A value of the Stern-Volmer constant (k(st)) for the homogeneous quenching of photoexcited Ru(bipy)(3)(2t) by Fe3+ has been obtained from electrochemical measurements conducted using the MORE. The value of k(st) so obtained (0.4 m(3) mol(-1)) compares favourably with the value of the Stern-Volmer constant for the same reaction obtained from fluorescence measurements (0.9 m(3) mol(-1))

Micro-optical ring electrode:Development of a novel electrode for photoelectrochemistry

The design of a novel photoelectrochemical sensor, the micro-optical ring electrode (MORE), is described, Based on a thin-ring microelectrode and using a fibre-optic light guide as the insulating material interior to the ring, the MORE is capable of delivering light directly to the region of electrochemical measurement and can therefore be used to conduct microelectrochemical studies of systems with complex photochemistry. A novel fabrication procedure is described, involving the coating of commercially available fibre optics (radius 1.25 x 10(-4) m) with a 600 nm layer of gold, so allowing exploitation of the electroanalytical advantages peculiar to thin-ring microelectrodes. The dark electrochemistry of the thin-ring microelectrode is characterized by use of cyclic voltammetry and chronoamperometry and found to agree with previously published theoretical results, Preliminary exploration of the photoelectrochemical response of the MORE is reported, achieved via the interrogation of the photoelectrochemically active phenothiazine dye methylene blue (MB(+)), Photocurrent signals obtained during cyclic voltammetric and chronoamperometric: studies of MB(+), conducted with the MORE under illuminated conditions and in the absence of any deliberately added reducing agent, are attributed to the formation and subsequent detection of (3)MB(+) within the diffusion layer of the microring electrode, The data demonstrate that the use of the MORE for the direct electrochemical detection of photogenerated species with lifetimes of <9 x 10(-5) s is possible, The electrochemistry of (3)MB(+) over the applied potential range from -0.4 to +1.0 V versus SCE is elucidated and discussed in the context of the behaviour of photoexcited MB(+) in the presence of the deliberately added reducing agent Fe2+